The Shiny (R package) application insane (INsulin Secretion ANalysEr) provides a web interactive tool to import experiments of insulin secretion using cell lines such as EndoC-βH1.
An Excel template is provided within the app to help users import their experiments in an easy way.
insane provides a user-friendly interface which can handle several projects separately.
insane performs technical quality-control of the optical density measured in each steps of the experiments:
This technical quality-control step checks:
insane performs statistical analyses of the experimental conditions, e.g., one silenced gene (siGENE) compared to an insulin secretion reference (siNTP) in two stimulation conditions (Glc and Glc + A).
Conditions are compared using a linear regression with
Operator as covariates (if needed) to control for heterogeneity.
Using all experiments in the selected project
Using some of the experiments in the selected project